关键词: 比格犬, 顺应性, SNP, 5-羟色胺, 酪氨酸羟化酶

Abstract: Objective　To initially establish a scoring standard on compliance for beagle dogs and explore the correlation between beagle dogs’ compliance and the c.264G>A SNP site of tyrosine hydroxylase (TH) gene and plasma 5-hydroxytryptamine (5-TH).Methods　By evaluating indicators such as the closeness between beagle dogs and humans, experimental cooperation, and convenience of feeding management, a preliminary scoring standard for beagle dogs’ compliance was established. Based on compliance assessment from 350 beagle dogs high and low compliance dogs (10 each) were selected.  The C264 SNP site of TH gene was analyzed by polymerase chain reaction sequencing method and plasma 5-HT concentration was determined by enzyme-linked immunosorbent assay kit. The correlation analyses were performed to explore the compliance with c.264G>A SNP site of TH gene and 5-HT concentration. Results　The dogs in the high compliance group were mostly GG genotypes according to the c.264G>A SNP site of TH gene and the 5-HT concentrations were higher [(32.73±8.64) mg/mL], while the dogs in the low compliance group were mostly AA genotypes and the 5-HT concentration were lower [(20.31± 6.99) mg/mL]. There was a significant correlation between compliance of beagle dogs and 5-HT concentration (P<0.05).Conclusion　The compliance of beagles shows a certain relationship with the c.264G>A SNP variation in the TH gene, although the difference is not statistically significant. However, it exhibits a significant correlation with plasma 5-HT concentration. Both the TH gene c.264G> A SNP and plasma 5-HT concentration may potentially serve as indicators and molecular markers for assessing tractability in beagles.

Key words: beagle dog, compliance, SNP, 5-HT, TH